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    ZymoBIOMICS RNA Miniprep Kit


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    Cat # Name Size
    R2001 ZymoBIOMICS RNA Miniprep Kit 50 Preps
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    Highlights

    • Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
    • Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
    • High Sensitivity: Increased detection limit of very low abundance organisms.
    Description & Documents Specifications FAQ Components Related Products

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    Supplemental Info

    Product Description

    The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.

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    Technical Specifications

    Equipment Microcentrifuge, vortex, cell disrupter (recommended)
    Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
    Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
    Size Range Total RNA ≥17 nt
    Yield 100 µg RNA (binding capacity), ≥50 µl (elution volume)
    Supplemental Info

    Resources

    Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA/RNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).

    Sample overloading can cause nucleic acids to precipitate and crash out of solution, reducing yields. Use less sample input to avoid precipitation.

    This is a normal occurrence as DNA/RNA Shield contains detergents. To reduce foam, centrifuge at 12,000 x g in 1 minute intervals before transferring the supernatant.

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Cat # Name Size
    D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml
    W1001-30 DNase/RNase-Free Water 30 ml
    R1100-50 DNA/RNA Shield 50 ml
    R1003-3-24 RNA Wash Buffer 24 ml
    R1060-1-50 RNA Lysis Buffer 50 ml
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    R1060-2-25 RNA Prep Buffer 25 ml
    C1001-50 Collection Tubes 50 Pack
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack
    E1010-1-4 DNA Digestion Buffer 4 mL
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes

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