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    Quick-DNA/RNA Viral 96 Kit


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    Cat # Name Size
    D7022 Quick-DNA/RNA Viral 96 Kit 2 x 96 Preps
    D7023 Quick-DNA/RNA Viral 96 Kit 4 X 96 Preps

    Highlights

    • Quick recovery of viral DNA/RNA from plasma, serum and other samples.
    • Omits the use of organic denaturants and proteases.
    • High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The Quick-DNA/RNA Viral 96 Kit is a fast viral DNA/RNA purification kit that provides rapid, large scale isolation of high-quality viral DNA and/or RNA from a wide range of biological sources. It can be used to successfully isolate viral DNA and/or RNA from cell-free body fluids, as well as cellular suspensions at concentrations ≤1x105 cells/ml. The kit has been rigorously tested and used to isolate viral RNA from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus and parvovirus (a ssDNA virus). The Quick-DNA/RNA Viral 96 Kit employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA adsorption onto the matrix of the Zymo-Spin I-96 Plate. The DNA/RNA is washed, then eluted with DNase/RNase-Free Water. The eluted DNA/RNA is suitable for use in various subsequent procedures including RT-PCR.

    Documents

    Technical Specifications

    Equipment Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
    Purity High-quality nucleic acids are ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.
    Sample Source Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab, and fecal samples
    Size Range 50 nt to ~200 kb
    Yield 5 µg DNA and 10 µg RNA
    Supplemental Info

    Resources

    The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.

    Yes, these samples are compatible.

    Yes, this kit will co-purify some DNA.

    Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).

    Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.

    The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.

    In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.

    For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.

    This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

    Cat # Name Size
    R1200-125 DNA/RNA Shield (2X Concentrate) 125 ml
    W1001-4 DNase/RNase-Free Water 4 ml
    W1001-10 DNase/RNase-Free Water 10 ml
    D7020-1-100 Viral DNA/RNA Buffer 100 ml
    C2002 Collection Plate 2 Plates
    C2004 Zymo-Spin I-96 Plate 2 Plates
    C2007-4 96-Well Plate Cover Foil 4 Foils

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