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Quick-DNA/RNA Viral 96 Kit
D7022 / D7023
Quick-DNA/RNA Viral 96 Kit
- Quick recovery of viral DNA/RNA from plasma, serum and other samples.
- Omits the use of organic denaturants and proteases.
- High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
The Quick-DNA/RNA Viral 96 Kit is a fast viral DNA/RNA purification kit that provides rapid, large scale isolation of high-quality viral DNA and/or RNA from a wide range of biological sources. It can be used to successfully isolate viral DNA and/or RNA from cell-free body fluids, as well as cellular suspensions at concentrations ≤1x105 cells/ml. The kit has been rigorously tested and used to isolate viral RNA from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus and parvovirus (a ssDNA virus). The Quick-DNA/RNA Viral 96 Kit employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA adsorption onto the matrix of the Zymo-Spin I-96 Plate. The DNA/RNA is washed, then eluted with DNase/RNase-Free Water. The eluted DNA/RNA is suitable for use in various subsequent procedures including RT-PCR.
|Equipment||Centrifuge with microplate carriers|
|Purity||High-quality nucleic acids are ready for Next-Gen sequencing, RT/qPCR, hybridization, etc.|
|Sample Source||Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab, and fecal samples|
|Size Range||50 nt to ~200 kb|
|Yield||5 µg DNA and 10 µg RNA|
Q1: Is the addition of beta-mercaptoethanol necessary?
The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.
Q2: Is this kit compatible with samples stored in UTM/VTM?
Yes, these samples are compatible.
Q3: Will this kit isolate DNA?
Yes, this kit will co-purify some DNA.
Q4: Can DNase I treatment be performed?
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow?
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added?
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
Q7: Why are my columns/plate wells getting clogged?
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
Q8: Why am I seeing delayed/no amplification during RT/qPCR?
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
|R1200-125||DNA/RNA Shield (2X Concentrate)||125 ml|
|W1001-4||DNase/RNase-Free Water||4 ml|
|W1001-10||DNase/RNase-Free Water||10 ml|
|D7020-1-100||Viral DNA/RNA Buffer||100 ml|
|C2002||Collection Plate||2 Plates|
|C2004||Zymo-Spin I-96 Plate||2 Plates|
|C2007-4||96-Well Plate Cover Foil||4 Foils|