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    ZymoBIOMICS Spike-in Control I (High Microbial Load)


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    Cat # Name Size
    D6320 ZymoBIOMICS Spike-in Control I (High Microbial Load) 25 Preps
    D6320-10 ZymoBIOMICS Spike-in Control I (High Microbial Load) 250 Preps

    Highlights

    • Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
    • in situ Quality Control: ensures each sample is quantified accurately
    • Unique composition: comprised of two microbes alien to the human microbiome
    Description & Documents Specifications FAQ Related Products

    Documents

    Supplemental Info

    Product Description

    ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.

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    Technical Specifications

    Biosafety This product is not biohazardous as microbes have been fully inactivated.
    Impurity Level < 0.01% foreign microbial DNA
    Reference Genomes Complete reference genomes and 16S rRNA genes
    Sample Storage -80°C
    Storage Solution DNA/RNA Shield
    Supplemental Info

    Resources

    Studies where strict bioburden is required, such as food or water studies, it would be useful to use the Spike-in Control.

    High biomass samples (feces, cell culture, etc.) should be used with Spike-In I.

    Low Biomass samples (swabs, water filters, etc.) should be used with Spike-In II.

    All bacterial sample types are compatible with the spike-in control.

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