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    Zymoprep-96 Yeast Plasmid Miniprep


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    Cat # Name Size
    D2005 Zymoprep-96 Yeast Plasmid Miniprep 2 x 96 Preps
    D2006 Zymoprep-96 Yeast Plasmid Miniprep 4 x 96 Preps
    D2007 Zymoprep-96 Yeast Plasmid Miniprep 8 x 96 Preps

    Highlights

    • Simple: Quickly and easily rescue plasmid from yeast.
    • Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
    • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.

    Documents

    Technical Specifications

    Applicable For Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
    Elution Volume ≥ 10 µl per well
    Equipment Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
    Processing Volume ≤ 1.5 ml of Culture
    Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
    Size Range Up to 25 kb.
    Yield Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

    Resources

    Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

    Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.

    Yes

    We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

    Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis

    Cat # Name Size
    D2004-1-45 Solution 1 Digestion Buffer 45 ml
    D2004-1-90 Solution 1 Digestion Buffer 90 ml
    D2004-2-45 Solution 2 Lysis Buffer 45 ml
    D2004-2-90 Solution 2 Lysis Buffer 90 ml
    D2004-3-180 Solution 3 Neutralizing Buffer 180 ml
    D2004-3-90 Solution 3 Neutralizing Buffer 90 ml
    D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml
    D4003-2-48 DNA Wash Buffer (Concentrate) 48 ml
    C1001-50 Collection Tubes 50 Pack
    C2002 Collection Plate 2 Plates
    C2003 Elution Plate 2 Plates
    C2004 Zymo-Spin I-96 Plate 2 Plates
    P1001-2 96-Well Block 2 Blocks
    C2007-8 96-Well Plate Cover Foil 8 Foils
    C2011-2 Air Permeable Sealing Cover 2 Pack
    C2011-4 Air Permeable Sealing Cover 4 Pack
    C2011-8 Air Permeable Sealing Cover 8 Pack

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