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    Zymoprep Yeast Plasmid Miniprep II


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    Cat # Name Size
    D2004 Zymoprep Yeast Plasmid Miniprep II 50 Preps

    Highlights

    • Simple: Quickly and easily rescue plasmid from yeast.
    • Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
    • High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
    Description & Documents Specifications FAQ Components

    Documents

    Product Description

    The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.

    Documents

    Technical Specifications

    Applicable For Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
    Elution Volume ≥ 10 µl
    Equipment Microcentrifuge & Heat Block/Bath
    Processing Volume ≤ 1.5 ml of Culture
    Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
    Size Range Up to 23 kb.
    Yield Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures

    Resources

    Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.

    Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.

    Yes

    We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.

    Any strains susceptible to Zymolyase, which includes: Susceptible fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis

    Cat # Name Size
    E1004 Zymolyase 1000 U
    D2004-1-10 Solution 1 Digestion Buffer 10 ml
    D2004-2-10 Solution 2 Lysis Buffer 10 ml
    D2004-3-20 Solution 3 Neutralizing Buffer 20 ml
    D4003-2-6 DNA Wash Buffer (Concentrate) 6 ml
    C1001-50 Collection Tubes 50 Pack
    C1003-50 Zymo-Spin I Columns 50 Pack

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