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ZymoPURE 96 Plasmid Miniprep Kit
D4214 / D4215
ZymoPURE 96 Plasmid Miniprep Kit
- High-Throughput: Perform 96 Plasmid Minipreps Simultaneously using a Vacuum Manifold or Centrifuge.
- Highest Yield: Up to 100 μg of Highly Concentrated Plasmid DNA.
- Ultra-Pure: Eluted Plasmid DNA is Transfection-Grade and Free of Cross-Contamination.
The ZymoPURE™ 96 Plasmid Miniprep Kit features a high-throughput method for the purification of up to 100 µg of ultra-pure transfection-grade plasmid DNA using a vacuum manifold or centrifuge. The unique vacuum plate design provides zero buffer retention and low elution volumes. In addition, the provided wash plate also significantly reduces plasmid DNA cross-contamination between wells relative to other popular high-throughput plasmid prep kits.
ZymoPURE™ technology uses a modified alkaline lysis method and our patented binding chemistry, which enables the highest DNA binding capacity and rapid loading of the lysate and wash buffer, resulting in the purification of highly concentrated (up to 1 µg/µl) plasmid DNA directly from a 96-well vacuum plate. In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR and other sensitive downstream applications.
As an added convenience, the ZymoPURE™ 96 Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. ZymoPURE™ Filter Plates are also included for rapid clearing of the lysate and the unique Zymo-Spin P-96 Plate design allows the binding step to be performed using a vacuum or centrifuge.
|Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
|≤ 5 mL
|≤ 1 EU/µg
|96-well plate vacuum manifold (recommended) or swinging bucket centrifuge with microplate adapters. The vacuum pump should be a single or double-staged unit capable of producing up to 300 mm Hg at the vacuum manifold.
|≤ 60 min
|Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0
|Recommended Sample Volume
|≤ 5.0 mL
|Up to ~200 kb
|Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.
Q1: What is the composition of the ZymoPURE™ Elution buffer?
10 mM Tris-HCL, 0.1 mM EDTA, pH 8.5
Q2: I ran out of ZymoPURE™ Wash 2. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Q3: Can an in-house vacuum line be used with the EZ-Vac Vacuum Manifold?
Yes, however, the pressure needs to be around 300 mm Hg. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.
Q4: Can the ZymoPURE Kits be used with other bacteria?
Yes, the standard protocol should work with other gram-negative bacteria. However, the user will need to validate the protocol for their particular species. For gram-positive bacteria, please refer to the Gram-Positive Bacteria Protocol in the Appendix of the kit protocol.
Q5: Can the ZymoPURE kits be used to isolate large plasmid constructs (BAC/PAC)?
Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase elution of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on column up to 10 minutes prior to centrifugation.
Q6: What vacuum manifolds is the ZymoPURE 96 kit compatible with?
This product is compatible with any commercially available 96-well plate vacuum manifold that can process stacked plates. We recommend our EZ-Vac 96 Vacuum Manifold (S7003).
Q7: What type of vacuum pump do you recommend?
The vacuum pump should be a single or double-staged unit capable of producing at least 300 mm Hg pressure at the vacuum manifold.
Q8: What are the dimensions of the 2.0 mL Deep Well Plate with the Zymo-Spin P-96 Plate on top?
128 mm x 86 mm x 76 mm. Ensure that the buckets on the rotor have sufficient clearance to accommodate this combo.
Q9: Is there a protocol for low-copy number plasmid DNA?
Increase the overnight culture processing volume up to 5 mL.
Q10: What is the recommended culture medium?
The plasmid purification protocol has been optimized for Luria-Bertani (LB) media. Enriched culture medias such as Terrific Broth or Super Broth can result in reduced performance and column clogging depending on the cell density and plasmid DNA copy number. Therefore, it might be necessary to reduce the volume of culture being processed when working with enriched culture media.
Q11: Do my samples have to be eluted in the provided 2.0 mL Deep Well Plate?
Other 96-well plates can be used for the elution step, but we highly recommend using the provided deep well plate to maximize the elution volume recovered and minimize cross-contamination. You may elute in a standard elution plate, but a lower vacuum pressure may be required (≤ 225 mm Hg).
|ZymoPURE P1 (Red)
|ZymoPURE P2 (Blue)
|ZymoPURE P3 (Yellow)
|ZymoPURE Binding Buffer
|ZymoPURE Wash 1
|ZymoPURE Wash 2 (Concentrate)
|ZymoPURE Elution Buffer
|ZymoPURE Filter Plate
|2 x 96-well plate
|Zymo-Spin P-96 Plate
|2 x 96-Well Plate
|2 x 96-Well Plate
|96-Well 2.0 mL Deep Well Plate
|Air Permeable Sealing Cover
|96-Well Plate Cover Foil