Tracking DNA Methylation in Breast Cancer at the Single-Cell and Spatial Levels
Featured Speaker
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Dr. Hisham MohammedAssociate Professor
Knight Cancer Institute at Oregon Health & Science University
Single-cell DNA methylation sequencing is transforming how we read tumor epigenomes. This webinar covers practical and analytical approaches for profiling methylation heterogeneity at single-cell resolution in hormone-driven breast cancer. We also explore the role of 'epimutations' and how they can be used to reconstruct lineage trees.
- How single-cell methylation profiling scales to thousands of cells, and what sample inputs and preparation it requires
- Analytical strategies for calling methylation states, defining cell populations, and integrating methylation with transcriptome and chromatin readouts
- How methylation heterogeneity tracks endocrine response, tumor evolution, and early transformation in breast cancer
Rewiring of regulatory elements is a key hallmark of breast cancer initiation and progression
Learn More About The Technologies in this Webinar
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EZ-96 DNA Methylation-Direct MagPrep
Compatible with small sample inputs – as few as 10 cells or 50 pg of DNA
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EZ-96 DNA Methylation-Lightning MagPrep
Uniquely gentle bisulfite chemistry with high recoveries.
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Zymo-Seq Trio WGBS Library Kit
Accurate Methylation Detection: Achieve precise methylation calling with a direct ligation-based protocol that preserves native termini, ensuring accuracy in each DNA fragment analysis.
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