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Read The Zymo Research PromiseZyppy Plasmid Miniprep Kit
| Cat # | Name | Size |
|---|
Highlights
- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
Documents
Product Description
Technical Specifications
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Equipment | Microcentrifuge and/or a vacuum manifold. |
| Processing Time | 8 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
Resources
Q1: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?
Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.
Q2: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?
≤ 50 Endotoxin Units/µg of plasmid DNA.
Q3: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?
No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.
Q4: Can the purified plasmid be transfected into eukaryotic cell lines?
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
Q5: What is the composition of the Zyppy Elution buffer?
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Q6: Can E.coli grown in enriched growth media be used with this kit?
Yes, pellet and resuspend E.coli in water or TE before proceeding with the protocol.
| Cat # | Name | Size | |
|---|---|---|---|
| C1019-50 | Zymo-Spin IIN Columns | 50 Pack | |
| C1001-50 | Collection Tubes | 50 Pack | |
| D4036-3-30 | Endo-Wash Buffer | 30 ml | |
| D4036-2-160 | Neutralization Buffer (Yellow) | 160 ml | |
| D4036-2-20 | Neutralization Buffer (Yellow) | 20 ml | |
| D4036-3-15 | Endo-Wash Buffer | 15 ml | |
| D4036-1-12 | 7X Lysis Buffer (Blue) | 12 ml | |
| D4036-1-48 | 7X Lysis Buffer (Blue) | 48 ml | |
| D4036-1-6 | 7X Lysis Buffer (Blue) | 6 ml | |
| D4036-2-40 | Neutralization Buffer (Yellow) | 40 ml | |
| D4036-3-120 | Endo-Wash Buffer | 120 ml | |
| D4036-4-12 | Zyppy Wash Buffer (Concentrate) | 12 ml | |
| D4036-4-48 | Zyppy Wash Buffer (Concentrate) | 48 ml | |
| D4036-4-6 | Zyppy Wash Buffer (Concentrate) | 6 ml | |
| D4036-5-20 | Zyppy Elution Buffer | 20 ml | |
| D4036-5-5 | Zyppy Elution Buffer | 5 ml | |
“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”
- Rebecca N.
“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”
- Richard F.
"The results gave what they promised! Clean DNA."
- Pamela R (UT Medicine San Antonio)
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