Direct-zol RNA Kits
The Direct-zol RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. With these RNA purification kits, total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.).
Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. These RNA purification kits use the Direct-zol™ method which assures unbiased recovery of small RNAs including miRNA (see below).
The procedure for these RNA purification kits is easy: simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. With these RNA purification kits, no phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
These RNA purification kits allow the entire process to be complete in only 7 minutes.
|Catalog #||Product||Binding Capacity||Elution Volume||Format||Cells||Tissue|
|R2061||Direct-zol RNA Microprep||10µg||6 µl||Spin-Column||≤ 1x106 Cells||≤ 5 mg Tissue|
|R2051||Direct-zol RNA Miniprep||50 µg||25 µl||Spin-Column||≤ 5x106 Cells||≤ 25 mg Tissue|
|R2071||Direct-zol RNA Miniprep Plus||100 µg||50 µl||Spin-Column||≤ 1x107 Cells||≤ 50 mg Tissue|
|R2055||Direct-zol-96||10µg||10 µl||96-Well Plate||≤ 1x106 Cells||≤ 5 mg Tissue|
|R2101||Direct-zol-96 MagBead||10µg||50 µl||Magnetic Beads||≤ 1x106 Cells||≤ 5 mg Tissue|
FOR UNBIASED LYSIS OF MICROBES AND/OR SOLID TISSUE IN TRIZOL, USE DIRECT-ZOL RNA WITH LYSIS TUBES.
$198.00 - $863.50
Direct-zol RNA Microprep Kits
R2060 / R2061 / R2062 / R2063
$204.60 - $872.30
Direct-zol RNA Miniprep Kits
R2050 / R2051 / R2052 / R2053