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    Direct-zol RNA Miniprep Plus Kits

    R2072 / R2071 / R2070 / R2073 / R2070T


    Direct-zol RNA Miniprep Plus Kits

    Cat # Name Size
    R2072 Direct-zol RNA Miniprep Plus 200 preps
    R2071 Direct-zol RNA Miniprep Plus (Product Supplied w/ 50 ml TRI Reagent) 50 preps
    R2070 Direct-zol RNA Miniprep Plus 50 preps
    R2073 Direct-zol RNA Miniprep Plus (Product Supplied w/ 200 ml TRI Reagent) 200 preps
    R2070T Direct-zol RNA Miniprep Plus 10 preps

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    Highlights

    • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
    • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
    • Non-Biased: Complete RNA recovery without miRNA loss.
    Description

    The Direct-zol™ RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

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    Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
    Equipment Microcentrifuge, vortex
    Sample Inactivation TRI Reagent® (provided with R2071, R2073) inhibits RNase activity and inactivates viruses and other infectious agents.
    Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
    Size Range Total RNA ≥ 17 nt
    Supplemental Info
    Yield 100 µg RNA (binding capacity), ≥ 50 µl (elution volume)

    Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

    All kit components are available for purchase separately.

    Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add an equal volume (1:1) of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

    Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

    Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

    Direct-zol DNA/RNA (R2080) kits can isolate DNA from TRIzol.

    Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

    The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

    Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

    Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

    Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Yes, the Direct-zol RNA kits are compatible with CTAB-based RNA extraction methods for polysaccharide-rich and/or phenolics-rich samples (e.g., Pinus, Geranium plants). Please find detailed protocol here.

    • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
    • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).




    “It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”

    - A. Newhart (The Wistar Institute)

    “Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”

    - Mohan K. (University of Illinois, Chicago)

    “Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”

    - Arjan V. (Indiana University)

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    Cat # Name Size
    E1010 DNase I Set 250 U
    R1003-3-12 RNA Wash Buffer 12 ml
    R1003-3-48 RNA Wash Buffer 48 ml
    R2072 Direct-zol RNA Miniprep Plus 200 preps
    R2070 Direct-zol RNA Miniprep Plus 50 preps
    C1006-50-G Zymo-Spin IIICG Columns 50 Pack
    C1001-50 Collection Tubes 50 Pack
    R2050-2-40 Direct-zol RNA PreWash (Concentrate) 40 ml
    R2050-2-160 Direct-zol RNA PreWash (Concentrate) 160 ml
    W1001-10 DNase/RNase-Free Water 10 ml
    W1001-30 DNase/RNase-Free Water 30 ml
    W1001-1 DNase/RNase-Free Water 1 ml
    E1010-1-4 DNA Digestion Buffer 4 mL
    R2050-1-200 TRI Reagent 200 ml
    R2050-1-50 TRI Reagent 50 ml
    E1010-1-16 DNA Digestion Buffer 16 mL