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    ZymoPURE - Express Plasmid Midiprep Kit


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    Cat # Name Size
    D4213 ZymoPURE - Express Plasmid Midiprep Kit 25 Preps.

    Highlights

    • Direct lysis protocol omits conventional cell-pelleting and resuspension steps.
    • Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
    • Prepare transfection-grade plasmid DNA in only 15 minutes.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE – Express Plasmid Midiprep kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of transfection-grade plasmid DNA directly from E. coli culture without conventional cell-pelleting and resuspension steps. The ZP Express Lysis Buffer is simply added directly to the culture and the plasmid DNA is purified following neutralization and removal of debris using the patented ZymoPURE binding system.

    The plasmid DNA is rapidly bound onto a column with a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The ZymoPURE – Express Plasmid Midiprep kit’s fast and simple protocol enables the recovery of highly concentrated transfection-grade plasmid DNA in just 15 minutes. As an added convenience, the ZymoPURE – Express Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. 

    Learn More

    Documents

    Technical Specifications

    Applicable For Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
    Binding Capacity 1.2 mg
    Culture Input 25 ml
    Elution Volume ≥ 150 µl
    Endotoxin Levels ≤1 EU/µg of Plasmid DNA
    Equipment Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
    Format Spin-Column
    Processing Time 15 min
    Purity Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0
    Recommended Sample Volume ≤ 25 ml
    Size Range Up to 200 kb
    Yield Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 25 ml of overnight culture grown in LB for high copy number plasmids are 100 – 200 µg.
    Supplemental Info

    Resources

    10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

    Yes, the RNase A is fairly stable at room temperature but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.

    The vacuum pump should be a single or double-staged unit capable of producing at least 400 mm Hg pressure at the vacuum manifold. If less pressure is applied, centrifuge the column prior to washing to remove any residual lysate/buffer remaining in the matrix.

    Yes, any vacuum that uses standard luer-lock connectors is compatible.

    Yes, however, the pressure needs to be around 400 mm Hg for the liquid to pass through the columns quickly. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.

    No, the ZymoPURE kits are only compatible with ZymoPURE Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE Wash 2 can be purchased separately.

    The binding capacity of the column is up 1.2 mg of plasmid, however, typical plasmid yields from directly processing 25 ml of overnight culture for a high copy plasmid is 100 – 200 µg.

    Yes, up to 50 ml of bacterial culture grown in LB can be pelleted, resuspended, and processed with the kit. Please see the appendix in the protocol for more information.

    Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase the elution efficiency of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on the column up to 10 minutes prior to centrifugation.

    Cat # Name Size
    D4213-1-210 ZP Express Lysis Buffer 210 ml
    D4213-2-260 ZP Express Neutralization Buffer 260 ml
    D4213-3-260 ZP Express Binding Buffer 260 ml
    D4200-5-20 ZymoPURE Wash 1 20 ml
    D4200-5-55 ZymoPURE Wash 1 55 ml
    D4200-5-320 ZymoPURE Wash 1 320 ml
    D4200-5-410 ZymoPURE Wash 1 410 ml
    D4200-6-10 ZymoPURE Wash 2 (Concentrate) 10 ml
    D4200-6-12 ZymoPURE Wash 2 (Concentrate) 12 ml
    D4200-6-23 ZymoPURE Wash 2 (Concentrate) 23 ml
    D4200-6-28 ZymoPURE Wash 2 (Concentrate) 28 ml
    D4200-7-1 ZymoPURE Elution Buffer 1ml
    D4200-7-6 ZymoPURE Elution Buffer 6 ml
    D4200-7-12 ZymoPURE Elution Buffer 12 ml
    D4200-7-30 ZymoPURE Elution Buffer 30 ml
    C1001-50 Collection Tubes 50 Pack
    C1001-500 Collection Tubes 500 Pack
    C1001-1000 Collection Tubes 1000 Pack
    C1092-5 ZymoPURE Syringe Filter-X 5 Pack
    C1037-5 ZymoPURE Syringe Plungers 5 Pack
    C1083-5 Zymo-Spin V-PS Column Assembly w/15 ml Reservoir-X and 50 ml Reservoir 5 Pack

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