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    Direct-zol RNA Miniprep Kits
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    Direct-zol RNA Miniprep Kits

    Direct-zol RNA Miniprep Kits

    Zymo Research's 100% Satisfaction Guarantee

    Satisfaction Guaranteed

    Read The Zymo Research Promise

    Cat # Name Size
    R2050 Direct-zol RNA Miniprep 50 preps
    R2051 Direct-zol RNA Miniprep (Product Supplied w/ 50 ml TRI Reagent) 50 preps
    R2052 Direct-zol RNA Miniprep 200 preps
    R2053 Direct-zol RNA Miniprep (Product Supplied w/ 200 ml TRI Reagent) 200 preps
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    Highlights

    • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
    • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
    • Non-Biased: Complete RNA recovery without miRNA loss.
    Description & Documents Specifications FAQ Components Citations

    Documents

    Supplemental Info

    Product Description

    The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

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    Documents

    Technical Specifications

    Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
    Equipment Microcentrifuge, vortex
    Sample Inactivation TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents.
    Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
    Size Range Total RNA ≥ 17 nt
    Yield 50 µg RNA (binding capacity), ≥25 µl (elution volume)
    Supplemental Info

    Resources

    All kit components are available for purchase separately.

    Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

    Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add an equal volume (1:1) of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

    Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

    Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

    If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

    Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

    Direct-zol DNA/RNA (R2080) kits can isolate DNA from TRIzol

    Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

    The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

    Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

    Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

    Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

    Input Average RNA Yield
    Cells 10 µg (per 106 cells)
    HeLa 15 µg
    High Yield Tissue (mouse) ≥ 30 µg (per 10 mg)
    Spleen 30-50 µg
    Liver 40-60 µg
    Low Yield Tissue (mouse) ≤ 30 µg (per 10 mg)
    Brain, Heart 5-15 µg
    Muscle 5-20 µg
    Lung 10-20 µg
    Intestine 10-30 µg
    Kidney 20-30 µg
    Whole Blood (per 1 ml)
    Porcine 10-20 µg
    Human 2-10 µg

    Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

    • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
    • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).

    Yes, the Direct-zol RNA kits are compatible with CTAB-based RNA extraction methods for polysaccharide-rich and/or phenolics-rich samples (e.g., Pinus, Geranium plants). Please find detailed protocol here.

    Cat # Name Size
    E1010-1-4 DNA Digestion Buffer 4 mL
    E1010-1-16 DNA Digestion Buffer 16 mL
    R2050-1-200 TRI Reagent 200 ml
    R2050-1-50 TRI Reagent 50 ml
    C1078-50 Zymo-Spin IICR Columns 50 Pack
    R1003-3-12 RNA Wash Buffer 12 ml
    R1003-3-48 RNA Wash Buffer 48 ml
    E1010 DNase I Set 250 U
    R2050-2-40 Direct-zol RNA PreWash (Concentrate) 40 ml
    R2050-2-160 Direct-zol RNA PreWash (Concentrate) 160 ml
    C1001-50 Collection Tubes 50 Pack
    R2050 Direct-zol RNA Miniprep 50 preps
    R2052 Direct-zol RNA Miniprep 200 preps
    W1001-6 DNase/RNase-Free Water 6 ml
    W1001-30 DNase/RNase-Free Water 30 ml

    “No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

    - Adina B. (University of Guelph)

    “This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

    - R.K. CSU

    “Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

    - H.Z. (Harvard Medical School)

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