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    ZymoBIOMICS 96 DNA Kit


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    Cat # Name Size
    D4303 ZymoBIOMICS 96 DNA Kit (Lysis Rack) 2 x 96 Preps
    D4307 ZymoBIOMICS 96 DNA Kit (No Lysis Matrix) 2 x 96 Preps
    D4309 ZymoBIOMICS 96 DNA Kit (Lysis Tubes) 2 x 96 Preps

    Highlights

    • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
    • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
    • Certified Low Bioburden: Boost your detection limit for low abundance microbes.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

    Learn More

    Documents

    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 20 µl DNA Elution Buffer or DNase free water
    Equipment Centrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
    Processing Volume Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)
    Purity High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
    Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
    Sample Storage Eluted DNA should be stored at ≤ -20°C.
    Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
    Type Total DNA
    Yield Up to 5 µg total DNA can be eluted into 20 µl
    Supplemental Info

    Resources

    Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).

    Check sample type and input amount. Low biomass samples such as swabs, some soil samples, water, air are expected to have low yield. High biomass samples such as feces may cause overloading of the spin column. Using less input may improve yields.

    Ensure sample is fully homogenized. Depending on bead beater used, the settings may have to be optimized for each sample. The homogenization time may be extended to see if yields improve.

    Apply heated elution buffer (60-70° C) and allow elution buffer to incubate on the column for several minutes prior to elution.

    Ensure that the DNA Elution Buffer is added directly to the column matrix.

    Low A260/230 values can be due to handling issues (e.g. transfer of column during wash steps). To minimize reagent wash buffer carryover, centrifuge the column at max speed for 1 minute prior to elution.

    Spin down lysate and Binding Buffer mixture at max speed for 5 minutes, this will eliminate any debris not caught by Spin III-F filter. Also avoid debris in lysis tube when transferring lysate, spin at max speed after bashing for 1-3 minutes.

    We have validated our kits with both high-speed homogenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficient (do not use adapters made of foam). You can reference the Optimized Lysis Protocols table under documents for some recommendations.

    This is to remove any debris from the lysate that could interfere with the DNA binding to the silica membrane of the column.

    Cat # Name Size
    D4300-4-200 ZymoBIOMICS DNA Wash Buffer 2 200 ml
    D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml
    D4302-4-200 ZymoBIOMICS MagWash 2 200 ml
    D4302-3-100 ZymoBIOMICS MagWash 1 100 ml
    D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml
    D4300-2-250 ZymoBIOMICS DNA Binding Buffer 250 ml
    D4300-3-100 ZymoBIOMICS DNA Wash Buffer 1 100 ml
    D4300-1-150 ZymoBIOMICS Lysis Solution 150 ml
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
    C2009 Silicon-A-HRC Plate 2 Plates
    S6002-96-3 ZR-96 BashingBead Lysis Rack 0.5 mm & 0.1 mm
    C2007-4 96-Well Plate Cover Foil 4 Foils
    C2004 Zymo-Spin I-96 Plate 2 Plates
    P1001-2 96-Well Block 2 Blocks

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