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    ZymoBIOMICS DNA Miniprep Kit


    Zymo Research's 100% Satisfaction Guarantee

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    Cat # Name Size
    D4300 ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) 50 Preps
    D4300T ZymoBIOMICS DNA Miniprep Kit 5 Preps
    D4304 ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) 50 Preps
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    Highlights

    • Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
    • Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
    • Certified Low Bioburden: Boost your detection limit for low abundance microbes.
    Description & Documents Specifications FAQ Components

    Documents

    Supplemental Info

    Product Description

    The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

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    Documents

    Technical Specifications

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 50 µl DNA Elution Buffer or DNase free water
    Equipment Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
    Processing Volume Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x109 bacterial, 2x108 yeast, or 2x107 mammalian cells)
    Purity High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
    Sample Source Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
    Sample Storage Eluted DNA should be stored at ≤ -20°C.
    Size Range Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
    Type Total DNA
    Yield Up to 25 µg total DNA can be eluted into ≥50 µl
    Supplemental Info

    Resources

    Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).

    Check sample type and input amount. Low biomass samples such as swabs, some soil samples, water, air are expected to have low yield. High biomass samples such as feces may cause overloading of the spin column. Using less input may improve yields.

    Ensure sample is fully homogenized. Depending on bead beater used, the settings may have to be optimized for each sample. The homogenization time may be extended to see if yields improve.

    Apply heated elution buffer (60-70° C) and allow elution buffer to incubate on the column for several minutes prior to elution.

    Ensure that the DNA Elution Buffer is added directly to the column matrix.

    Low A260/230 values can be due to handling issues (e.g. transfer of column during wash steps). To minimize reagent wash buffer carryover, centrifuge the column at max speed for 1 minute prior to elution.

    Spin down lysate and Binding Buffer mixture at max speed for 5 minutes, this will eliminate any debris not caught by Spin III-F filter. Also avoid debris in lysis tube when transferring lysate, spin at max speed after bashing for 1-3 minutes.

    We have validated our kits with both high-speed homogenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficient (do not use adapters made of foam). You can reference the Optimized Lysis Protocols table under documents for some recommendations.

    This is to remove any debris from the lysate that could interfere with the DNA binding to the silica membrane of the column.

    Cat # Name Size
    C1058-50 Zymo-Spin III-HRC Filters 50 Pack
    S6012-50 ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) 50 Tubes
    C1078-50 Zymo-Spin IICR Columns 50 Pack
    D4300-1-40 ZymoBIOMICS Lysis Solution 40 ml
    D4300-2-100 ZymoBIOMICS DNA Binding Buffer 100 ml
    D4300-3-50 ZymoBIOMICS DNA Wash Buffer 1 50 ml
    D4300-7-30 ZymoBIOMICS HRC Prep Solution 30 ml
    D4300-4-60 ZymoBIOMICS DNA Wash Buffer 2 60 ml
    D4302-5-1 ZymoBIOMICS DNase/RNase Free Water 1 ml
    D4302-5-10 ZymoBIOMICS DNase/RNase Free Water 10 ml
    C1057-50 Zymo-Spin III-F Filters 50 Pack

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